Place it at the end of a misunderstanding scene, just after the mistaken object is shown, so the denial closes the joke. Follow it with a Bruh sound for disbelief, or open with an Oh My God meme reaction before the reveal to build up the confusion.
About this sound
This is a short Dutch spoken clip, about six seconds long, in which a voice delivers the line 'En nee die is geen joint' with a flat, matter-of-fact tone. It feels dry and deadpan, like a calm correction that lands funnier because it sounds so certain. It comes from the Ad Looij meme, where the line is used as a blunt denial.