Place it at the start of the interruption beat, before any response from the person being stopped. Follow it with the bruh soundboard button for the culprit's reaction, then cut back to your main clip so the exchange reads as call-out, reaction, and move on.
About this sound
This is a short 1.8-second voice clip of a voice politely saying 'Excuse me sir,' tagged as Mobility Mary. The delivery feels prim, insistent and a little awkward, which makes the interruption land as funny rather than rude. It leaves listeners with that caught-off-guard, trying-not-to-laugh feeling of being stopped mid-stride by someone who absolutely wants your attention.