The sound comes from Fallout's Vault-Tec Assisted Targeting System, the slow-motion aiming mode first introduced in Fallout 3 and carried through later games in the series including Fallout 4 and Fallout: New Vegas. In gameplay it confirms that an enemy or object has been selected for a targeted shot, which is why players associate it so strongly with aiming and decision-making. Clips of the interface spread through gameplay videos and meme edits, and the short lock tone became shorthand online for picking a target, making it a favourite for creators outside the games themselves.