Put it at the exact cut near the end of a horror edit, after a short warning cue and before the reaction shot. Open with the Danger Alarm Meme Sound to set up the moment, then let this clip hit, and follow it with the FNAF Jumpscare Scream for a second hit.
About this sound
This is a short jumpscare sound lasting just under a second, made to deliver a sudden scare in a single burst. It lands abruptly and gives a jolt of shock and unease, the tense fright that makes people flinch and laugh nervously after the surprise. It is tied to Five Nights at Freddy's and its sudden jumpscare moments.