Place it at the climax of a horror edit, after Dun Dun Dun has set up the reveal and just before it lands. Follow it with Fortnite Death Sound to mark the defeat, or with SpongeBob Fail if the scare is meant to turn comic.
About this sound
This is a short jumpscare effect from FNAS 5, lasting about two seconds and built to hit without warning. It feels sudden, tense and startling, the kind of burst that makes a quiet moment snap into panic. It comes from the fan-made Five Nights at Sonic's series, where it marks the moment a character catches the player.