Place this clip at the punchline, after your main gag has landed. Follow it with the Vine Boom Sound Effect for a bigger reveal, or swap that order and end a failed attempt with the SpongeBob Fail Sound Effect instead.
About this sound
This clip is a short vocal sting lasting just under six seconds, built around a single abrupt exclamation rather than a full line of dialogue. It feels awkward and absurd in a funny way, turning a normal moment tense for a split second before the laugh lands, which is why it works as instant reaction punctuation.