Put it at the end of a setup shot, right on the visual gag, then follow it with Nelson Ha Ha for a mocking laugh, or cut to Brother Eww for a disgusted reaction. Keep it as the punchline in an edit and pair it with one reaction sound only, so the joke stays quick and clear.
About this sound
A two-second toot that sounds deliberately squeaky and cartoon-like, more like a comic sound effect than a real bodily noise. It feels playful and goofy, giving a listener a quick jolt of surprise that turns an ordinary moment into something childish and absurd. It works as a tiny, unexpected interruption for a joke, an awkward pause, or a harmless prank.