Place it at the end of a build-up, after a Metal Gear Alert that warns something is about to go wrong, then follow it with an Evil Laugh when the rival realises the mistake. That order turns a small setback into a full dramatic showdown, with the alert setting the stakes and the laugh closing the scene.
About this sound
This is a very short, under-two-second angry vocal reaction from Goku, the hero of Dragon Ball, delivered as a sharp burst of frustration rather than a line of dialogue. It feels tense and suddenly serious, like a calm character snapping, and that quick shift makes a small annoyance seem huge and funny in a meme edit.