Place it at the end of a short reaction sequence, after a SpongeBob Fail sting sets up the embarrassment and before a Vine Boom Sound Button hit closes the scene for a bigger second beat.
About this sound
This clip is a short, exaggerated silly laugh lasting just under two seconds, performed in a high, wobbly voice that sounds deliberately cartoonish. It gives a listener a feeling of goofy disbelief, turning an awkward or absurd moment into something playful rather than mean, and making the joke feel lighter and more ridiculous.