Place it at the end of a failed joke or awkward exchange, just before the scene changes, then follow it with a reaction such as Bruh or Goofy Ahh Sounds to widen the joke without crowding the moment.
About this sound
This clip is a single, very short human grunt repeated twice, lasting under half a second, with a flat, throaty delivery and no other words or music. It lands as awkward and blunt, like a shrug in sound form, funny because it says so little while still sounding unimpressed. It works best as a quick reaction to something strange, silly or hard to answer.