Use it just as the failed attempt peaks, then follow with the Fail Sound Meme for a second beat after the flop. For a slower build, open with It Was At This Moment He Knew, then switch to this clip as the payoff.
About this sound
A four-second cut of the Harry Potter theme turns a well-known wizard tune into a quick comic sting. It feels funny because the sweeping, spell-like music lands on something small and clumsy, making an ordinary slip feel like a failed spell. The melody is Hedwig's Theme from the films, introduced in 2001 and closely tied to the series ever since.