Place it right after the fail lands, as the closing beat of the joke, then follow it with bruh for a deadpan beat or Emotional Damage when the loss stings more. Keep it as the final reaction so the next clip can start clean.
About this sound
This two-second clip is the King's rhythmic chuckle from Clash Royale, blown out with crunchy deep-fried distortion that makes it harsher and more absurd than the clean game audio. It feels deliberately smug and annoying, turning a small slip into a joke at the listener's expense. It comes from the King's laughing emote in Supercell's mobile game, released in 2016.