Place it just after the offending line lands, then follow it with Oh My God Wow for a bigger reaction or Eww Brother Eww when the next line is even grosser, keeping this clip as the first beat and the second clip as the escalation.
About this sound
This is a short, roughly two-second spoken Japanese phrase, 'hidoi naa', delivered as a wounded reaction to something unfair or unkind. It lands with playful hurt and mock disbelief, the kind of exaggerated complaint that turns a cold remark into a joke without losing its sting.