Place it right at the escalation point in your edit, after the calm setup and before the reaction shot. Follow it with a Yeah Reaction Sound Button moment for agreement, or precede it with a Metal Gear Solid Alert to flag the sudden shift in stakes.
About this sound
This is a half-second burst taken from Hoobastank's rock track 'Out of Control', cut down to a sharp musical hit with no room to build. It feels frantic and overcommitted, like the exact second a casual contest stops being casual and someone decides winning matters more than looking calm or in control.