The phrase comes from Sweet Bro and Hella Jeff, the intentionally crude comic within Andrew Hussie's webcomic Homestuck, which began in 2009. In the strips, Sweet Bro warns Hella Jeff about stairs, and Hella Jeff keeps falling down them anyway, turning the warning into a running catchphrase. Fans quoted the awkward phrasing across forums and image boards, and the voice clip later became shorthand online for any ignored warning that plays out exactly as predicted, especially around falls and fails.
About this sound
The 'I warned you about the stairs bro' sound effect from Homestuck's Sweet Bro and Hella Jeff, used as a deadpan warning before an obvious fail.
It is a short, flat delivery of the line 'I warned you about the stairs bro,' spoken like a friend who already knows how this ends. The voice stays calm and almost bored, which makes the warning funnier than a shout would be. There is no build-up, just the deadpan payoff right as someone ignores advice and heads for disaster. Played over a fall, a bad decision, or a gaming wipe, it turns a simple mistake into a running joke about being told exactly what would happen and doing it anyway.