Place it right after the embarrassing reveal, then show the reaction shot. Pair it with the Bruh Meme Sound before the moment for a double take, and follow it with the Fail Sound Meme once the joke lands to underline the defeat.
About this sound
This is J. Jonah Jameson's sharp, booming laugh from Spider-Man, lasting about eight seconds and delivered in the bossy, overbearing style of the Daily Bugle editor. It feels mocking and gleefully self-satisfied, like a boss enjoying someone else's embarrassment. The laugh comes from the live-action Spider-Man films, where J.K. Simmons plays Jameson.