Place it at the peak of a near-miss, right after sudden suspense builds the tension and just before Nelson's ha-ha seals the joke, so the refusal becomes the payoff of the whole sequence.
About this sound
This is a short clip of JonTron shouting the line I'm not gonna die with rising, panicked energy, lasting just under five seconds. The delivery flips fear into bravado, so it lands as funny and defiant rather than serious, perfect for a moment when someone refuses to give in. It works best as a quick burst of over-the-top confidence.