Place it at the cut where someone is stopped mid-request, then follow it with Bruh Meme for the reply, and put It Was At This Moment He Knew just before it to frame the block as a bad decision.
About this sound
The clip is a single sharp 'Ni!' shouted by one of the Knights Who Say Ni, lasting under a second. The clipped, demanding delivery feels absurd and playful, turning a tiny word into a mock-serious threat. It comes from Monty Python and the Holy Grail, where the knights block travellers until they bring a shrubbery.