Place it at the cut where a character makes an impossible demand, then follow it with a shocked gasp and a short laugh track to sell the interruption, before returning to the scene with a calm line that restores the flow.
About this sound
This three-second clip is the Knights Who Say Ni chanting their strange, demanding 'Ni' in unison, a short vocal gag with a sharp, theatrical delivery. It feels absurd and funny, like a sudden interruption that turns a normal scene into nonsense. It comes from the 1975 film Monty Python and the Holy Grail, where the knights confront King Arthur.