Place this clip right after the mistake or reveal, then follow it with the Anime Ahhh Sound Button for a second reaction beat. For a flatter punchline, swap that follow-up for the Awkward Moment Sound Button and let the scene move on without another effect layered underneath.
About this sound
This clip is Kronii from Hololive delivering a long, drawn-out 'Oh Fhhgyaaa' in a high, flustered voice that stretches for almost six seconds. It feels panicked and funny at once, like a brain short-circuiting after something embarrassing or unexpected, which is why listeners use it as an exaggerated overreaction rather than a normal scream.