Place it after the setup shot and before the reaction close-up so the joke lands in sequence. Follow it with a bruh sound effect for a deflated contrast, or precede it with a scream meme sound when building toward a bigger, layered meltdown in longer edits.
About this sound
The clip is just over a second of a stretched-out vocal wail, delivered with an exaggerated, strained tone that lands all at once. It feels frantic and over-the-top, turning shock, frustration or sudden surprise into something absurd rather than serious, which is why that single drawn-out cry reads instantly as panic played for laughs.