Place it near the end of a night sequence, after the X-Files Theme has set the mood, then follow it with Awkward Crickets to undercut the moment with a joke.
About this sound
This clip is a long wolf howl lasting about 34 seconds, built around one drawn-out awooo cry that holds its wild, animal tone throughout. It feels eerie and unsettling, like a lone wolf calling across dark open ground, and it leaves a tense, spooky stillness behind it.