Place it in the middle of an edit as the pivot point, after a gentle opening and before the scare. Build tension first with Inception Horn Sound Effect, then follow it with FNAF Jumpscare Scream to land the payoff in the next beat.
About this sound
This is LilyPichu performing an exaggerated demonic laugh lasting about seven seconds, shifting from a playful giggle into a harsh, cackling roar. The sudden change from her soft voice to something sinister feels both amusing and uncomfortable, like a joke that has gone wrong. It comes from the streamer herself, and her gentle on-screen persona makes the outburst land harder.