Place this after the build-up line and before your reaction shot, so the entrance carries the cut. Pair it with the Prowler sound effect for a villain reveal sequence, or follow it with the Miguel O'Hara theme when the edit continues into a full Spider-Verse montage.
About this sound
This is a 2.4-second bass-boosted sting built around Miguel O'Hara, the Spider-Man 2099 character, with the low end pushed up for extra weight. It feels tense, stern and instantly intimidating, like a warning that someone serious has just arrived and the fun part of the scene is over.