Put it in the middle of an edit, after a light, familiar meme cue and just before the reveal image, then follow it with a sudden impact sound once the disturbing image is on screen.
About this sound
This is the second short audio cue from the Mr. Incredible Becoming Uncanny meme, lasting about four and a half seconds with a dark, horror-style tone and no dialogue. It feels tense and deeply unsettling, like a normal scene suddenly turning wrong. It comes from the viral format using increasingly disturbing still images of the character from The Incredibles, where each stage uses darker audio to signal a deeper descent.