Place it immediately after the failed joke in your edit, then follow it with Wrong Answer Game Show to underline the miss, and finish with Nelson's Ha-Ha! for a second reaction from another character.
About this sound
This four-second clip is a single spoken Portuguese line, 'No man, you're not funny,' delivered in a flat, unimpressed voice with no music or effects behind it. It lands as blunt and awkwardly funny, the kind of deadpan shutdown that makes a bad joke feel even worse, and it works best as a quick, cutting reply.