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Place it at the climax of a build-up, after the frustrating moments have stacked up, then follow it with a funny ha ha laugh to close the sequence. For a sharper payoff, put a yeah reaction just before it, so the agreement comes first.
About this sound
This is a nearly nine-second Portuguese voice clip delivering the line about no human being able to stand something, spoken with dramatic, exasperated emphasis. It feels overwhelmed and funny at once, like a breaking point reached in public, and it lands best as an over-the-top reaction to something absurd, irritating or impossible to endure.
