Place it as the closing beat of a fail edit, after the mistake is already clear. Start with the Roblox Oof Sound Button for the hit itself, then follow with this clip to end the sequence, and leave any reaction shot for after it finishes.
About this sound
This clip is a short musical game over sting, about four and a half seconds long, played when a run ends in defeat. It feels deflating and a little funny, turning a small mistake into something final and unmistakable. It comes from New Super Mario Bros for the DS, where it plays when Mario loses his last life.