Put it near the start of an anime montage as the opening gag, then follow it with Mayuri Tuturu for a second idol-style reaction before the main clip begins. For a bigger payoff, close the sequence with JoJo To Be Continued after the reveal lands.
About this sound
The clip is Nico Yazawa's bright, sing-song 'Nico Nico Nii' catchphrase, delivered in about four and a half seconds with a playful, idol-like flourish. It feels cheeky, upbeat and deliberately cute, like a character striking a pose just to get a reaction. It comes from Love Live! School Idol Project, where Nico uses it as her signature greeting.