The exact source of this no, of course not clip is not documented, which is common for short reaction lines pulled from television dialogue and shared out of context. Its power is in its universality: the phrase is a classic English sarcastic denial, instantly understood without setup. On soundboards it sits alongside other deadpan no and really reaction clips, and short-form creators reach for it because the sub-second length fits perfectly over captions, stitches and duets where a longer rant would kill the pacing.
About this sound
A short sarcastic voice clip saying no, of course not in a dismissive tone. Creators use it as a reaction sound to deny the obvious or mock a silly suggestion.
This is a one-second spoken reaction clip of someone saying no, of course not with flat, slightly exasperated delivery. The voice is calm and dry rather than shouted, with a small pause before of course that sells the sarcasm. It lands like a deadpan eye-roll in audio form, instantly shutting down a ridiculous question or an obvious suggestion. In edits it works as a punchline button, making the viewer feel the creator saw the bad take coming and dismissed it without effort.