Place this clip right after the claim you are answering, as the response shot in the edit. For a longer bit, lead in with the you what spongebob sound to show confusion first, then use this denial as the final answer before cutting away.
About this sound
Sandy Cheeks snaps out 'No, you ain't!' in a quick 1.3-second voice clip, her Texas drawl putting extra weight on the last word. It feels blunt, teasing and instantly defiant, like a friendly challenge rather than a polite correction. The line comes from SpongeBob SquarePants, where Sandy is known for her confident, straight-talking attitude.