Place Dun Dun Dun just before this clip to build suspense, then use this clip for the reveal itself, and finish with Vine Boom after the reaction lands to close the sequence with a joke.
About this sound
This clip is a single loud scream lasting about four seconds, raw and unbroken throughout. It hits with sudden shock, then stretches the tension just long enough to feel dramatic rather than comic, making a listener flinch before they laugh. The sound works as a pure reaction, sharp, urgent and impossible to ignore.