Place it right after a sudden suspense sting when the spider appears on screen, then follow it with the Eww Brother Eww reaction for a second beat, or use Oh My God Wow instead if the scene needs a bigger second reaction.
About this sound
This is a short Polish spoken reaction lasting about three and a half seconds, built around the exclamation “O kurde, tu są pajączki!”, or “oh damn, there are little spiders here!” The speaker sounds suddenly startled by something small and creepy nearby, turning a moment of disgust into something comic. It feels panicky, squeamish and funny at once, perfect for a jump-scare reaction.