Place it at the turning point of a fail edit, after the setup and before the consequence lands. Follow it with Emotional Damage Meme for the aftermath, or open with Oh My God Meme to set up the moment before this clip begins.
About this sound
This five-second clip is a single panicked voice repeating oh no again and again, starting worried and then speeding up and rising in pitch until the words tumble into a breathless string of oh no no no no no. It feels frantic and funny at once, like watching a small slip turn into a full disaster, and it makes the listener brace for whatever goes wrong next.