Place it right at the reveal, after a moment of confusion built with You What, then follow the scream with My Leg for a second SpongeBob gag. Open with Law and Order Dun Dun if you want the reveal staged as a courtroom twist before the joke lands.
About this sound
This is Patrick Star letting out a short, high-pitched scream of about one second, shouting that the figure in front of him is no lady. The sudden burst feels panicked and ridiculous, turning a mistaken identity into an instant joke. It comes from SpongeBob SquarePants, where Patrick's over-the-top reaction makes the moment land as pure cartoon chaos.