Place it immediately after the accusation in your edit, so the denial answers the claim without a gap. Follow it with No No Wait Wait for a panicked backtrack, or cut to Oh My God Wow when the other person doubles down instead of accepting it.
About this sound
This two-second voice clip catches a man firmly denying words put in his mouth, repeating that he did not say 'tat' in a defensive, slightly flustered tone. It feels instantly funny and awkward, like being caught mid-argument and doubling down, which makes it perfect for playful denials and exaggerated misunderstandings.