Place it at the peak of a reaction edit, after the setup line and just before the cutaway. Follow it with the Rizz Meme Sound Effect to keep the same character on screen, or open with the Dramatic Dun Dun Dun Sound Effect and let this clip close the stare.
About this sound
This is a brief dramatic cue, just over a second long, built around a sharp hit that lands like a spotlight on a single look. It feels suspicious and smugly confident, turning an ordinary glance into a challenge. It comes from the Dwayne Johnson stare meme, where the sound is timed to his lifted eyebrow and a sudden close-up on his face in short-form reaction edits.