Use it as the bridge between a setup shot and the payoff shot, then follow it with a fail sound meme for a heavier landing or clapping for a mock celebration. Keep it as the only accent in that beat so the next gag still lands cleanly.
About this sound
This is a single slide whistle glissando that climbs quickly and then drops straight back down, lasting just over a tenth of a second. It feels light, cheeky and immediately comic, like a visual gag translated into sound. The recording comes from the 1953 Donald Duck short The New Neighbor and was later released in the Hollywood Edge effects library.