Place this clip at the point where a plan visibly fails, after building tension with sudden suspense and before a reaction. Follow it with no no wait wait to extend the scramble, keeping this as the main turning point in the sequence.
About this sound
An almost 15 second voice clip of Spider-Man realising his webs are not working properly, starting in a polite, matter-of-fact tone before stretching the final word into a long, shouted DANGERRRRR. The shift from calm problem-solving to outright panic is what makes it funny, turning a superhero failure into an exaggerated, helpless overreaction.