Place it right after the mistake lands in your edit, then follow it with the funny laugh to turn the embarrassment into a joke, and use the alert sound just before it to signal that trouble is coming.
About this sound
This eight-second clip holds two long, drawn-out human screams, each stretching a groaning wail into an exaggerated cry. The extended delivery makes it feel desperate, ridiculous and oddly dramatic at once, like someone reacting far beyond what the moment deserves. It works as pure exaggerated frustration.