Place it right after the noisiest exchange in your edit, then follow it with a Vine Boom to underline the reset, and put a Dramatic Dun Dun Dun before it when you want a bigger build-up.
About this sound
This nearly six-second clip lets a tense mood build before a strained, angry voice barks out the demand for silence twice, making a plain request sound like a stagey shutdown. The result feels both funny and commanding, because the abrupt order stops the noise and pulls every ear toward the speaker. It has spread online as a reaction clip passed between soundboards and short edits, with no scene behind it.