Place it at the end of a failed attempt, after a short build-up, so the reaction closes the scene. Put Dun Dun Dun just before it to raise the stakes, then follow with SpongeBob Fail as the next beat.
About this sound
This five-second voice clip features Boomer delivering the line in an exaggerated, despairing tone, stretching a small disappointment into a full-blown catastrophe. It feels melodramatic and funny, like a cartoon tantrum, and comes from his reaction to learning he would not be voiced by Greg Cipes in the Powerpuff Girls reboot.