Put it at the peak of a scare sequence, after a tense build and just before the reveal, then follow it with the FNAF Jumpscare Scream for a second hit that keeps the reaction going.
About this sound
This is a short, remastered jumpscare burst lasting just under two seconds, built around a harsh, distorted scream and sudden crashing noise. It hits fast and leaves a jolt of shock and panic, the kind that makes a listener flinch before they can laugh it off. It comes from the Five Nights at Freddy's jumpscare sound.