Place it just after the suspicious glance is established, then follow it with a cartoon boing and a quiet laugh, keeping it as the middle beat that turns the scene from normal to absurd.
About this sound
Twiddlefinger is a roughly 26-second meme audio clip with an odd, playful tune that feels deliberately offbeat. It gives a scene an awkward, goofy edge, like something slightly unhinged is happening, and cues viewers that the moment is not meant to be taken seriously. It works best when a glance, a fidget or a sudden weird turn needs an instant unserious signal.