Place it just after the chase begins, then follow it with Run Vine as the pursuit speeds up and Enemy Spotted when the follower is finally seen. That order keeps the joke moving from question to chase to reveal without crowding the scene.
About this sound
This is a short Russian spoken line asking why someone is running after the speaker, delivered in just over two seconds. The direct question lands as confrontational and a little comical, like catching someone in the act and calling them out on the spot. It works best as a quick retort, with the chase itself doing most of the comic work.