Place it at the exact moment a mistake is revealed, before any reaction or commentary, so the warning lands first. Follow it with the Classic Error Sound Effect for a bigger blunder, or the Bell Ding Sound Effect when the error is small and playful, and save the Dramatic Dun Dun Dun Sound Effect for the reveal that follows.
About this sound
This is the two-note exclamation chime from Windows XP, lasting barely a tenth of a second, with a warm, rounded electronic ring. It feels polite yet urgent, like a gentle nudge that something needs attention, and it sparks recognition for anyone who used a PC in the 2000s. Microsoft included it in the default sound scheme of the operating system, released in 2001, where it marked warnings and blocked actions.