Place it immediately after the Chamber misplay in your edit, once the failed play is clear to viewers. Open the round with The Bomb Has Been Planted to set the stakes, then follow this question with Nope when the loss is confirmed.
About this sound
A 2.7-second Japanese voice line puts a direct question to Chamber about deranking, using the competitive-gaming term for deliberately dropping rank. It feels skeptical and teasing rather than angry, like catching a teammate out after a strangely poor round. The line is aimed at Chamber, the Valorant agent, which gives it a clear ranked-play edge.