Put it at the end of a setup clip, just as the action fails, then follow it with the Bruh sound for a flat reaction, or cut to the Game Show Buzzer when the mistake is a wrong answer.
About this sound
This two-second clip is a heavily distorted brake screech, clipped and crunchy until the sound breaks apart, with the sharp squeal of brakes pushed too far. It feels abrupt and jarring, like a sudden halt after a rush of speed, and lands as a comic shock rather than a smooth stop.