This sound comes from Cuphead, the run-and-gun game known for its 1930s cartoon presentation and announcer-style voice clips. Short lines from the game spread online because they are clean, dramatic and easy to drop into edits without much setup. “You’re up” stayed useful because it clearly marks a handoff, a turn or the start of pressure. That makes it a natural fit for gaming compilations, tournament posts, reaction clips and meme edits where someone is suddenly put on the spot.