Place it at the end of a failed attack sequence, after Enemy Spotted and just before Roblox Oof, to turn one loss into a short chain of game defeats for viewers.
About this sound
This is a 0.31-second female death cry from Age of Empires II, a sharp, cut-off vocal sound with no music or other effects. It lands as sudden and grimly comic, the kind of abrupt loss that makes a game moment feel instantly worse. It comes from the game's villager death sounds, the fourth female variant in the set.